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International Journal of Virology

Year: 2012  |  Volume: 8  |  Issue: 2  |  Page No.: 191 - 202

Partially Nucleotide Sequence and Secondary Structure of Chrysanthemum stunt viroid Egyptian Isolate from Infected-Chrysanthemum Plants

K.A. El-Dougdoug, A.A. Rezk, Dawoud A. Rehab and A.R. Sofy

Abstract

Chrysanthemum stunt viroid Egyptian isolate (CSVd-EG) was isolated from infected Chrysanthemum plants. It is a member of Pospiviroidae. In order to study the structure of CSVd-EG, it was reverse transcribed in total RNA from infected leaves and then amplified by polymerase chain reaction (PCR) using Pospiviroid-CCR specific primers. Purified gel RT-PCR product (~199) was cloned into the PCR® II-TOPO® vector then it was sequenced. Partial sequence 199 bp of CSVd-EG is almost identical to that of the prototype 199 bp Canada and USA isolates of CSVd with 96% homology. The sequence of CSVd-EG can be arranged into viroid specific rod like structure. CSVd-EG differ from the prototype isolates Canada and USA at sites occur in regions corresponding to the conserved, variable and right terminal domains which are believed to control viroid pathogencity. Finally, this constitutes the first isolation and identification of CSVd from diseased Chrysanthemum plants in Egypt.

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