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International Journal of Virology

Year: 2012  |  Volume: 8  |  Issue: 1  |  Page No.: 106 - 113

Biological and Molecular Characterization of Potato Infecting Alfalfa Mosaic Virus in Egypt

H.S. El-Helaly, A.A. Ahmed, M.A. Awad and A.M. Soliman


Alfalfa Mosaic Virus (AMV) was isolated from naturally infected potato (Solanum tuberosum, L. cv. Spunta) plants, showing bright yellow blotching or mottling Calico and aucuba symptoms suspected to be due to virus infection. Leaf samples were collected from different localities in Minufyia Governorate, Egypt. AMV was readily mechanically inoculated by sap extracted from infected potato leaves to host plants (bioassay). The identity of AMV isolate was confirmed by sequence analysis of their Coat Protein (CP) gene. The sequence of the Coat Protein gene (CP) of AMV was determined from cDNA clones. The CP gene was cloned into pGE®-T Easy vector and transformed into Escherichia coli strain DH5α. The recombinant plasmids were obtained and sequenced. The nucleotide sequences were compared with corresponding viral nucleotide sequences reported in GenBank. The analysis showed that nucleotide sequence of the Egyptian isolate of AMV (GenBank Accession No. HQ288892) had percentage of similarity ranged from 97-98% with eight reported AMV isolates. Thus, a method of identification and detection by RT-PCR of AMV was established.

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