Influence of Soybean Mosaic Virus Infection on Carbohydrate Content in Nodule of Soybean (Glycine max L. Merr.)
The present investigation was conducted to see the effect of Soybean Mosaic Virus (SMV) infection on carbohydrate content in nodules of soybean. Observation made on the carbohydrate (reducing sugar, non-reducing sugar and starch) content of nodules in soybean mosaic virus infected soybean indicated the reduction of all the contents in comparison to nodules in the healthy plants. Reducing sugar ranged from 2.0 to 3.39 mg g-1 dry weight in healthy and in diseased it ranged from 1.86 to 3.00 mg g-1 dry weight and in non reducing sugar ranged from 1.4 to 2.75 mg g-1 dry weight and in healthy and in diseased it ranged from 1.3 to 2.45 mg g-1 dry weight. The results were significant in the case of non-reducing sugar. The reducing and non-reducing sugars were estimated by the colorimetric method. The alcohol extract was clarified and residue left on the filter paper was returned to the extracting flask, dried at 65°C and preserved for starch analysis.
Cited References Fulltext