Biological, Serological and Molecular Diagnosis of Three Major Potato Viruses in Egypt
Viruses are major diseases affecting potato plantations in Egypt. Different molecular and serological methods for virus detection were used to detect the Potato virus X (PVX), Potato virus Y (PVY) and Potato leaf roll virus (PLRV). ELISA, RT-PCR, immunocapture RT-PCR (IC-RT-PCR) and RT-PCR-ELISA were the detection methods used. Naturally virus-like infected potato samples were collected from different locations at El-Munofia Governorate in Egypt. PVX and PVY were isolated and identified biologically through host range and indicator plants. All detection methods mentioned above were sensitive enough to detect the three potato viruses in potato tissues. Using ELISA method, PVX, PVY and PLRV were detected in 4, 7 and 10 out of 26 samples (15.4, 26.9 and 38.5%), respectively. Mixed infection was found between PVX/PVY; PVY/PLRV and also among PVX/PVY/PLRV. According to the results, it is the use of molecular methods to confirm certain serological results i.e., the virus especially when low concentration in the infected samples. Using the advantage of IC with RT-PCR-ELISA will reduce the costs and avoid RNA degradation. Additionally, RT-PCR-ELISA could be the method of choice among the molecular methods.
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