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International Journal of Virology
Year: 2009  |  Volume: 5  |  Issue: 4  |  Page No.: 164 - 169

Detection of Hepatitis B Polymerase Gene in Early Embryonic Cells from Golden Hamster Oocyte and Human Spermatozoa Carrying HBV DNA

B.A. Ali, H.H. Salem, X.M. Wang, T.H. Huang, Q.D. Xie and X.Y. Zhang    

Abstract: The study on vertical transmission of hepatitis B Virus DNA in the human embryo would be an ideal model but such a system presents major logistical, moral and ethical problems. Thus, it is crucial to establish a model system for such study. The present study was designed to amplify HB polymerase gene in one and two cell embryo originated from hamster ova in vitro fertilized with human spermatozoa carrying HBV-DNA. Human sperm carrying HBV-DNA was delivered into zona-free hamster oocytes by in vitro fertilization assay. HB polymerase gene which is responsible for the reverse transcription of the pregenomic RNA to the double stranded DNA has been detected both in one- and two-cell embryos using PCR. The results reveal that HB polymerase gene is present in one- and two-cell embryo (single embryo) originated from hamster ova in vitro fertilized with human spermatozoa carrying HBV DNA sequences. Present results provided the direct evidences that HB-DNA could transmit vertically to next generation via male germ line.

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