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International Journal of Virology
Year: 2009  |  Volume: 5  |  Issue: 3  |  Page No.: 131 - 142

Etiology of a Mosaic Disease of Radish and Lettuce and Sequencing of the Coat Protein Gene of the Causal Agent in Saudi Arabia

M.A. AL-Saleh, I.M. AL-Shahwan, M.A. Amer and O.A. Abdalla    

Abstract: This study aimed at identifying the causal agent inducing virus-like symptoms on radish and lettuce plants in Riyadh Region, Saudi Arabia. Mottling, chlorosis and mosaic symptoms were observed on lettuce (Lactuca sativa Linn.) and radish (Raphanus sativus Linn.) plants in two areas in Riyadh region. Mosaic symptoms were observed on L. sativa, R. sativus, Eruca sativa Mill and Brassica rapa Linn., whereas local lesions were observed on Chenopodium ammaranticolor Coste and Reyn., which were mechanically inoculated with sap from infected radish and lettuce plants. Electron microscopy revealed filamentous flexuous particles typical of potyviruses. The aphid Brevicoryne brassicae transmitted the virus to lettuce and radish in a non-persistent manner. Turnip mosaic virus (TuMV) was the only detected virus by double antibody sandwich-enzyme linked immunosorbent assay among five viruses suspected of such disease symptoms. Amplification of total RNA extracted from infected lettuce and radish plants yielded 1-1.2 kbp using three degenerate primers which were designed for potyvirus group detection and 985 bp complementary DNA (cDNA) fragments using a specific oligonucleotide primer for TuMV detection. Nucleic acid sequence analysis of TuMV-L-SA revealed a range of 85.3 to 90.9% sequence identity with other TuMV isolates obtained from the GenBank. However, the TuMV-Ra-SA revealed a range of 87.7 to 94.1% sequence identity. The sequence similarity between TuMV-L-SA and TuMV-Ra-SA investigated in the current study was 89%. To our knowledge, this is the first report of TuMV on lettuce and radish in Saudi Arabia.

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