Asian Science Citation Index is committed to provide an authoritative, trusted and significant information by the coverage of the most important and influential journals to meet the needs of the global scientific community.  
ASCI Database
308-Lasani Town,
Sargodha Road,
Faisalabad, Pakistan
Fax: +92-41-8815544
Contact Via Web
Suggest a Journal
International Journal of Virology
Year: 2005  |  Volume: 1  |  Issue: 1  |  Page No.: 3 - 3

Cloning, exprision and nucleotide sequence of coat protein gene of an egyptian isolate of potato virus Y strain NTN infecting potato plants

M.A.Amer, M. H. El-Hammady, H.M. Mazyad, A. A.Shalaby and F.M. Abo-El- Abbas    

Abstract: The coding sequence of coat protein gene of an Egyptian isolate of PVY was modified to has an initation AUG code for translation. Reverse Transcription-Polymerase Chain Reaction (RT-PCR) amplified product of an expected size of 801 bp, cloned into the Pin Point Xa-1 protein expression vector. The fidelity of each PCR amplified PVY-CP gene was tested by PCR, restriction analysis and translation. Analysis by in vitro translation using western blotting assay on nitrocellulose membrane using monoclonal antibodies verified that the PVY-CP gene correctly encoded and expressed a protein reacting with PVY antibodies. The CP gene ( 32 KDa) was reacted successfully with PVY specific monoclonal antibodies in dot blot immunobinding assay ( DIBA) as well as in western blot assay. The nucleotide sequence of the Egyptian isolate of PVY CP gene was determined to be 801 nucleotides in length encoding a deduced amino acid. Nuelcotide sequence analysis revealed a range of 96- 99.5% sequence identity among the PVY NTNisolates.

Cited References   |    Fulltext    |   Related Articles   |   Back
  Related Articles

Copyright   |   Desclaimer   |    Privacy Policy   |   Browsers   |   Accessibility