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International Journal of Tropical Medicine
Year: 2007  |  Volume: 2  |  Issue: 2  |  Page No.: 74 - 77

A Comparison Between Replication of Rinderpest Virus-Saudi 1/81 Virulent Strain in B95a and MoMo Cell Lines

M. Haroun, I. Hajer, M.M. Mukhtar, S.A.M. Kheir and B.E. Ali    

Abstract: In vitro cultivation of rinderpest virus is a routine procedure to isolate the virus in B95a cell lines. Monocyte modified human cell lines (MoMo) were not known to be used commonly for cultivation of Rinderpest Virus (RPV). In this study the ability of both cell lines to support replication of RPV was tested. B95a and MoMo cell lines sustained replication of RPV-Saudi 1/81 virulent strain. Classical RPV Cytopathic Effect (CPE) was observed in both cell lines starting at day 5-6 of co- cultivation with Peripheral Mononuclear Cells (PBMC) of the infected calves. RPV P-protein antigen was detected in both infected cell lines on day 4 of inoculation.

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