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International Journal of Poultry Science
Year: 2014  |  Volume: 13  |  Issue: 2  |  Page No.: 114 - 123

Actigen® Influence on the Gene Expression of Heat Shock Proteins in Ross 708 Broiler Chickens

Z.S. Lowman, F.W. Edens, C.M. Ashwell and S.J. Nolin    

Abstract: In field trials, heat-exposed chickens given Actigen®, a second generation mannan oligosaccharide (MOS) from Saccharomyces cerevisiae, maintained good intestinal health and performance. This investigation explored the influence of Actigen® on heat shock protein (HSP) responses in Ross 708 broiler chickens. Gender-segregated broilers were given either a control or Actigen®-supplemented (800 g/ton in starter, 400 g/ton in grower and 200 g/ton in finisher) diet over a 6 week growing period. At 3 and 6 weeks of age, broilers of each gender on each diet were exposed to 41°C for 1 h in a temperature-controlled chamber while controls were maintained at 24°C. After heat exposure, liver and ileum tissues were collected and preserved in RNAlater for determination of gene expression via Real Time PCR. Significant differences in mRNA expression for HSP90A, HSP90AA and HSP90B due to gender were found in the ileum, but no gender-related differences for these HSPs were found in the liver. In all heat-exposed birds, gene expression was elevated for HSP90A, HSP90AA, HSP90B, HSP70 and HSP60 in both liver and ileum with males at 3 and 6 weeks of age showing the greater HSP response. Lower Actigen®-related HSP90AA and HSP90B mRNA expression in the liver suggested that Actigen® potentially modified HSP expression outside the intestinal tract. Actigen® mechanism (s) of action outside the intestine are equivocal, but they might be indirect. Lower HSP mRNA expression in Actigen®-fed birds indicated that the supplement can modify the HSP response while allowing continued good performance during heat-exposure.

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