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International Journal of Poultry Science

Year: 2004  |  Volume: 3  |  Issue: 8  |  Page No.: 547 - 549

Carrier State Studies of Infectious Bronchitis Virus in Asymptomatic Layer in Pakistan

M.S. Mahmood, M. Siddique and I. Hussain


Isolation of infectious bronchitis virus (IBV) was conducted in asymptomatic layer flocks with a history of IB or IB vaccination at least from 5 to 20 weeks before the day of sampling. A trypsin-induced hemagglutination (THA) assay to detect IBV in allantoic fluid of embryonated eggs was used. Detection of IBV through THA showed that group A [5 wks post-inoculation (PI)] was 100%, group B (10 wks PI) was 86%, group C (15 wks PI) was 74% and group D (20 wks PI) was 28% positive for IBV. Virus titre was found negatively correlated with the PI duration.

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