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International Journal of Poultry Science
Year: 2004  |  Volume: 3  |  Issue: 8  |  Page No.: 547 - 549

Carrier State Studies of Infectious Bronchitis Virus in Asymptomatic Layer in Pakistan

M.S. Mahmood, M. Siddique and I. Hussain    

Abstract: Isolation of infectious bronchitis virus (IBV) was conducted in asymptomatic layer flocks with a history of IB or IB vaccination at least from 5 to 20 weeks before the day of sampling. A trypsin-induced hemagglutination (THA) assay to detect IBV in allantoic fluid of embryonated eggs was used. Detection of IBV through THA showed that group A [5 wks post-inoculation (PI)] was 100%, group B (10 wks PI) was 86%, group C (15 wks PI) was 74% and group D (20 wks PI) was 28% positive for IBV. Virus titre was found negatively correlated with the PI duration.

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