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International Journal of Plant Pathology
Year: 2014  |  Volume: 5  |  Issue: 3  |  Page No.: 70 - 83

Molecular Identification and Analysis of Coat Protein Gene of Cucucmber mosaic cucumovirus Sugar Beet Egyptian Isolate

A.A. Megahed, Kh.A. El- Dougdoug, B.A. Othman, S.M. Lashin, M.D. Hassanin, M.A. Ibrahim and A.R. Sofy    

Abstract: Cucumber mosaic cucumovirus beet Egyptian isolate (CMV-Beet-EG) was detected serologically by DAS-ELISA using polyclonal antibodies in naturally infected sugar beet plants. The virus was isolated by single local lesion method on Chenopodium amaranticolor and then, propagated on sugar beet plants. The virus isolate was confirmed serologically by using dot and tissue printing immunoassay. The coat protein gene of CMV-Beet-EG was successfully amplified using specific primer by RT-PCR and the expected size was 820 bp. Sequence analysis cp gene revealed 97.3 and 96.4% identified similarity with CMV strains sequences published in GeneBank. The highest content of nitrogen bases was for Thymine (T) 164 (26.5%) followed by Cytosine (C) 163 (26.3%), then Adenine (A) 148 (23.9%) and Guanine (G) 144 (23.3%). The ratio of A/T and G/C were 0.902 and 0.883, the percentage of A+T and G+C was found 50.4 and 49.6. The deduced amino acids sequence of CMV-Beet-EG/CP gene was 206 amino acids with a molecular weight of 22.983 kDa and isolelectric point of 9.95. There are total 20 amino acids, the amino acid Serine (S) has the highest content and ferquency of all amino acids 25 and 12.1% while the lowest content and frequency of amino acid was 1 and 0.5% to the each of amino acid Histidine (H) and Trpyptophan (W). The CMV-Beet-EG/CP gene sequence was recorded in GeneBank with Accession No. JX826591.

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