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International Journal of Plant Breeding and Genetics
Year: 2011  |  Volume: 5  |  Issue: 1  |  Page No.: 76 - 85

Effect of Sodium Azide on the Growth and Variability Induction in Helianthus annuus L.

Gehan G. Mostafa    

Abstract: Seeds of Helianthus annuus cultivars Giza 1 and 102 were soaked in sodium azide solutions with the concentrations of 0, 100, 200, 300, 400 and 500 ppm for 4 h. This research aimed to study the effect of sodium azide on the growth of sunflower and induction of genetic variation in the vegetative and flowering growth as new patterns. The two genotypes Giza 1 and 102 produced a wide range of response to sodium azide concentrations by the studied traits. Concerning to the genetic variations, the changes of leaf and inflorescence form were found. Dwarfed and variegated plants were also found. Plants with many inflorescences were obtained and transmitted to next generation. An attempt was done to propagate the variegated plant in the tissue culture laboratory. Shoots were obtained from nodes cultured on MS medium supplemented with 0.05 BA and 0.05 IBA. These shoots failed to root in MS medium supplemented with 0.1 IBA.

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