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International Journal of Pharmacology
Year: 2020  |  Volume: 16  |  Issue: 3  |  Page No.: 223 - 235

Stem Cell Mobilization with G-CSF and Cyclophosphamide Ameliorated Collagen-Induced Arthritis in Wistar Rats

Mohamed L. Salem, Enas A. Abdul Baki, Abdel-Aziz A. Zidan and Rehab M. Elgharabawy    

Abstract: Background and Objective: Rheumatoid arthritis is an inflammatory autoimmune disorder which affects multiple joints and causes cartilage erosion. It’s treatment depends on the systemic use of anti-inflammatory and immunosuppressive drugs. The aim of this study was to test effect of combination of Granulocyte-Colony Stimulating Factor (G-CSF), cyclophosphamide (CTX) and Bone Marrow (BM) cells on Collagen Induced Arthritis (CIA) in Wistar rats. Materials and Methods: Collagen Induced Arthritis (CIA) was induced in Wistar rats by subcutaneous (s.c.) injection of200 and 100 μL/rat of collagen II at tail base in on days 0 and 12, respectively. Then, rats were treated with either intravenous injection of fresh Bone Marrow (BM)cells(5×106) or s.c. injection ofG-CSF(5 μg for s.c. for 5 consecutive days)in presence or absence of a single intraperitoneal injection of cyclophosphamide (4 μg/rat). Inflamed paws, x-ray and histopathological examination were assessed during the experiment. Serum levels of IL-4, IL-8 and TNF-α were also measured.Results: All treatments decreased inflammation in the paws as reflected by decreased paw thickness in fore and hind limbs and confirmed by x-ray and histological examination. Of note, the anti-CIA effects after treatment with CTX plus G-CSF were higher than of CTX plus BM cells and other treatment modalities (CTX or BM alone). Interestingly, all treatment modalities induced decreases in levels of IL-4, IL-8 and TNF-α. Among them, CTX/G-CSF showed the highest inhibitory effect on TNF-α.Conclusion: Treatment of CIA rats with the combination of G-CSF and CTX induces potential anti-arthritic effect. This anti-arthritic effect is through down regulating the elevated levels of IL-4, IL-8 and TNF-α cytokines.

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