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International Journal of Pharmacology

Year: 2017  |  Volume: 13  |  Issue: 1  |  Page No.: 22 - 32

Protective Effect of Vitazinc on Chlorsan Induced Oxidative Stress, Genotoxicity and Histopathological Changes in Testicular Tissues of Male Rats

A.A. Khalaf, M.A. Ibrahim, A.F. Tohamy, A.A. Abd Allah and Amr R. Zaki

Abstract

Background: Chlorsan is highly effective bactericide and fungicide, so it is being widely used as insecticides in Egypt and the potential toxicity was raised more attention as it caused an oxidative stress and genotoxic effect in testicular tissue. Vitazinc, one of the most effective antioxidant and may play a role on preventing the toxic effect. Materials and Methods: Fourty mature Albino rats were divided into four groups (10 rats each). Group (1) control rats received orally an equivalent volume of corn oil on body weight. Group (2) rats in this group were orally administrated 1/10 LD50 of chlorsan equal to 1 mg kg–1 b.wt., dissolved in corn oil for 60 days. Group (3) male rat in the groups were orally administrated 1/10 LD50 of chlorsan in dose level of 1 mg kg–1 b.wt., beside 200 mg kg–1 b.wt., vitazinc. Group (4) rats in the group received orally 200 mg kg–1 b.wt., vitazinc only. Results: Chlorsan caused significant increase in lipid perioxidation. While significant inhibition in the activity of catalase (CAT), ChE activity and decrease in the level of reduced glutathione (GSH) were evident. Genotoxicity results revealed significant increase in the amount of m RNA of COX-2 and significant increase in the values of DNA fragmentation. Interestingly, pretreatment with Vitazinc attenuated these adverse effects. Vitazinc, therefore is a potent antioxidant and can protect against chlorsan-induced oxidative damage and genotoxicity by reducing lipid peroxidation and enhancing the antioxidant defense mechanisms. Histopathological examination revealed marked changes in testes of male treated rats. Conclusion: The present study reveals that vitazinc is effective in attenuating the oxidative stress, genotoxicity inflicted by chlorsan toxicit.

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