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International Journal of Pharmacology
Year: 2014  |  Volume: 10  |  Issue: 3  |  Page No.: 168 - 174

Strain Typing and Strain Differentiation of Mycobacterium leprae by TTC Repeats

Santosh Chokkakula, Kalyani Dasari, Idress Hamad Attitalla, Srikantam Aparna, Padmaja Ponnada and Madan Mohan Male    

Abstract: The main pathogen based molecular markers involved in strain typing and strain differentiation are SNP and VNTR. A typing system for global differentiation of M. leprae is the analysis of variable number tandem repeats based on STRs. To expand the analysis of M. leprae TTC gene polymorphism, this study involved strain typing of 14 Lepromatous Leprosy (LL) SSS samples. For the strain typing and strain differentiation of M. Lprae, the present study involves 14 bacteriological positive Lepromatous Leprosy (LL) Slit Skin Smear (SSS) samples. Most of cases were identified as male and 13 cases have associated with nerve involvement. Two cases with tenderness and two with eye involvement also have been screened. DNA was extracted from SSS of 14 untreated LL leprosy cases. PCR amplification of TTC gene and sequencing of amplicons showed the allelic variation and percentage of distribution of allele in the enrolled samples. Among the 14 SSS samples, 1 (7%) exhibited 9 copy number, 1 (7%) associated with 10 copy number, 6 (43%) identified as 12 copy number, 1 (7%) known as 13 copy number, 1 (7%) exhibited 15 copy number, 1 (7%) linked with 16 copy number, 1 (7%) exhibited 18 copy number and 2 (15%) identified as 19 copy number of (TTC) 21 allele. However, the positive strain called TN was associated with standard repeat units of TTC that is 21. As conclusion we found that, the present results has demonstrated that TTC repeats of leprosy patients has 12 and 19 copy number as a abundant which was slight differ from other leprosy strains from India.

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