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International Journal of Pharmacology

Year: 2011  |  Volume: 7  |  Issue: 6  |  Page No.: 710 - 715

Determination of the Expression of COX II and Aromatase Protein in Parent and Tamoxifen-resistant Subline of Human Breast Cancer T47D Cells

S. Fouladdel, E. Azizi, H.R. Rahimi and S.N. Ostad

Abstract

Genetic differences among tumor cells of breast cancer patients are the main reasons for therapeutic failure. COX-2 and aromatase proteins have been determined to be important in breast cancers as potential targets for prevention and can also be a part of the therapeutic regimen of these cancers. Consequently, we decided to determine the expression of these proteins in human breast cancer T47D cells and its established tamoxifen resistant subline, namely T47D/TAMR-6 cells. Immunocytochemical technique was employed using primary antibodies for each protein followed by visualization of results with LSAB2 detection kit under the microscope. Our data showed that expression of COX-2 was relatively the same in parent and resistant T47D cells in the presence and absence of Tamoxifen (1 uM). Unlike tamoxifen, celecoxib could dramatically decrease the expression level of COX-2 in both cell types. Aromatase protein expression seems to be absent or is expressed at very low levels in both cell types and under all experimental conditions. Our data indicates no significant difference between studied cell types with respect to expression of aromatase and COX-2 enzymes. Therefore, these data suggest different possible outcomes for specific inhibitors of these enzymes alone or in combination with tamoxifen in the therapeutic regimen of breast cancer patients.

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