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International Journal of Pharmacology
Year: 2011  |  Volume: 7  |  Issue: 1  |  Page No.: 125 - 129

In vivo Antinociceptive Activity of Leaf Extract of Crinum asiaticum and Phytochemical Analysis of the Bioactive Fractions

M.Z. Asmawi, O.M. Arafat, S. Amirin and I.M. Eldeen    

Abstract: Crinum asiaticum is a perennial bulbous herb native to tropical South-eastern Asia. The plant is useful in Asian and African traditional medicine as an analgesic agent. This report aimed to investigate the antinociceptive effects of leaf extracts of Crinum asiaticum on rats and to highlight the major chemical groups of the bioactive fractions. Powdered leaf of Crinum asiaticum was sequentially extracted using petroleum ether, chloroform and methanol. The extracts were investigated for their antinociceptive activity on rats using the carrageenan-induced paw hyperalgesia method. Pain threshold response was measured by applying pressure on the rat's paw using analgesy-meter. Oedema was induced by injection of 0.1 mL 1% (w/v) carrageenan dissolved in normal saline into the footpad of the right hind paw of the rat and allowed to develop. The test samples were administered orally in a dose of 1000, 500 and 250 mg kg-1 (10 mL kg-1). Rats administered orally with vehicle were used as negative group. Indomethacin (1 mg kg-1) was used as positive control. After 4 h, the pain threshold of each rat was measured. The best activity was shown by the chloroform extracts at the dose of 250 mg kg-1 (n = 4-5 in each group; p<0.05), followed by the methanol extracts. No activity was observed with the petroleum ether extract (1000 mg kg-1). Indomethacin was used as positive control (1 mg kg-1). Phytochemical screening of the active fractions indicated the presence of alkaloids, coumarins, glycosides, triterpenes and flavonoids. The results obtained support the reported uses of the plant as traditional analgesic agent.

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