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International Journal of Pharmacology
Year: 2010  |  Volume: 6  |  Issue: 4  |  Page No.: 431 - 434

Effects of Scrophularia striata Ethanolic Leaves Extracts on Staphylococcus aureus

A.M. Bahrami and Valadi Ali    

Abstract: The in vitro antibacterial activity of ethanolic leaf extract of Scrophularia striata alone and in combination with antibiotics (doxycycline and ofloxacin) by means of Fractional Inhibitory Concentration Indices (FICI) as well as by the use of time-kill assays Gram-positive bacterium (Staphylococcus aureus). Antibacterial activity was assayed by using the microdilution method. The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were determined for the ethanolic leaf extract of Scrophularia striata alone and also in combination with antibiotics using the fractional inhibitory concentration (FIC) and time-kil assay method. Synergism was also tested using checker board dilution method. MIC/MBC values for ethanolic leaf extract of Scrophularia striata against all the tested bacteria ranged between 25.5-52.6/22.4-60.5 μg mL-1, for doxycycline 4.0/4.0-4.5 μg mL-1 and for ofloxacin 0.625-2.5/1.25-5.0 μg mL-1, respectively. The average log reduction in viable cell count in time-kill assay ranged between 2.4-4.5 log10 cfu mL-1 after 1 h of interaction and between 3.9-5.0 log10 cfu mL-1 after 3 h interaction in 1xMIC to 4xMIC. When leaf extract and antibiotics were combined, the average log reduction in viable cell count for doxycycline from 1.5-5.18 log10 cfu mL-1 and for ofloxacin 3.06-5.39 log10 cfu mL-1.

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