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International Journal of Pharmacology

Year: 2008  |  Volume: 4  |  Issue: 6  |  Page No.: 460 - 465

Alpha Mannosidase Inhibitory Effect of Some Iranian Plant Extracts

A. Gholamhoseinian, H. Fallah, F. Sharifi-Far and M. Mirtajaddini

Abstract

Alpha 1,2-mannosidase is a key enzyme in N-glycan processing in the endoplasmic reticulum (ER) and Golgi apparatus, have been one of enzyme targets in the development of anti cancer therapies. One hundred species of plants with known and unknown medicinal properties were collected and botanically identified. Methanolic and aqueous extracts prepared by maceration method. Enzyme inhibitory effects against a-mannosidase was determined spectrophotometrically at pH 4.5 and 25 °C using 0.5 mM p-nitrophenyl- α-D-mannopyranoside as the substrate and 1 units mL-1 Jack bean alpha mannosidase in 0.02 M citrate buffer. Among 200 extracts, ten extracts showed more than 20% inhibitory activity on alpha mannosidase; Punica grantum, Damask rose and Quercus infectoria among them showed more than 40%. The kinetic study of the enzyme showed that the inhibition mechanism of the three more active extracts were non competitive. Under the control condition Km value for the enzyme was 1.59 mmol and Vmax was 0.039 mmol min-1 Vmax in presence of 4 μg mL-1 Punica grantum, Damask rose and Quercus infectoria extracts were 0.020, 0.022 and 0.025 mmol min-1, respectively. The data indicated that these plants are good candidates for therapeutic use and deserve to purify the active agents effective against α- mannosidase. Further in vitro and in vivo studies are needed to reveal the actual effectiveness of each of them.

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