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International Journal of Pharmacology
Year: 2005  |  Volume: 1  |  Issue: 4  |  Page No.: 332 - 335

Determination of Rofecoxib in Serum with Pre-column Derivatization and Fluorescence Detection

Mohsen Amini, Morteza Pirali-Hamedani, Hossein Ali Ebrahimi, Majid Darabi and Abbas Shafiee    

Abstract: A HPLC method for determination of rofecoxib in human serum was presented. The method is based on pre-column derivatization of analyte to a phenanthrene derivative of drug. Rofecoxib and internal standard were extracted from serum using liquid-liquid extraction. Upon exposure to UV light, drug was found to undergo a phtocyclization reaction giving a species with high absorbance. Validation of the method has been studied in the concentration range of 2-100 ng mL-1.

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