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International Journal of Dairy Science

Year: 2020  |  Volume: 15  |  Issue: 4  |  Page No.: 169 - 181

Ameliorative Role of Ashwagandha/Probiotics Fortified Yogurt against AlCl3 Toxicity in Rats

Mohamed A. Mustafa, Mahmoud Ashry, Heba H. Salama, Samy M. Abdelhamid, Laila K. Hassan and Khaled G. Abdel-Wahhab


Background and Objective: Yogurt is a distinctive vesicle for active compounds and the widest-spread fermented dairy product. This study aimed to explore the ameliorative role of emulsified yogurt fortified with Ashwagandha Ethanolic Extract (AEE) and probiotic bacteria against Aluminium Chloride (AlCl3)-induced toxicity in rats. Materials and Methods: Yogurt was evaluated chemically, microbiologically and sensory as well as biologically using experimental animals. Results: Results revealed that fortified yogurt with either AEE, probiotics, or their mixture did not disturb the main chemical composition of yogurt. Yogurt antioxidants and phenolic content increased by adding AEE, alone or in the presence of probiotics; however, it decreased after 15 days of storage but remained higher in the mixture-treated yogurt. Furthermore, all the physicochemical and sensory properties were significantly improved with the addition of AEE, separately or combined with probiotics bacteria. The biological study showed oral administration of rats with the emulsions of yogurt fortified with AEE and probiotic bacteria, alone or in combination, together with Aluminium chloride succeeded to decline AlCl3-induced toxicities; this was evidenced by the significant reduction in serum tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL1β), alanine aminotransferase (ALAT), aspartate aminotransferase (ASAT), urea, creatinine, cholesterol, triglycerides and low dense lipoprotein-cholesterol (LDL-c) values. Also, reduced glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT) were markedly increased in liver, kidney and brain tissues coupled with a sharp reduction in the malondialdehyde (MDA) and nitric oxide (NO). The neurochemical markers, dopamine, serotonin and acetylcholinesterase (ACh-ase) were also favorably improved. Conclusion: It could conclude that AEE and probiotics succeeded to improve physicochemical, microbiological, sensory qualities as well as health benefits as they restored AlCl3-induced hepato-renal-neuro deteriorations; they are promising-supplement for the protection against toxicities.

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