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International Journal of Dairy Science
Year: 2017  |  Volume: 12  |  Issue: 2  |  Page No.: 105 - 113

Identification of Staphylococcus aureus Causing Bovine Mastitis using MALDI-TOF Fingerprinting

Waleed S. Shell, M. L. Sayed, Aatia A. El-Gedawy, Ghada M. El Sadek, A. A. Samy and Abdelhakam M.M. Ali    

Abstract: Background: Reliable and rapid methods for identification of clinical bacterial isolates are mainly dependent on phenotypic and genotypic characteristics of the bacteria. As an alternative identification methods, mass spectral (proteomics) analysis for identification of clinical bacterial isolates including Staphylococcus aureus has been recognized. This study was aimed to evaluate and compare the performance, reliability and sensitivity of conventional bacteriology, phenotypic methods and MALDI-TOF MS in identification of clinical Staphylococcus aureus isolated from bovine mastitis cases. Materials and Methods: Ninety five milk samples were collected from three dairy farms (Giza governorate-Egypt) with high incidence of contagious bovine mastitis and examined for isolation and detection of the causative agents using classical identification, API-Staph kit and matrix-assisted laser desorption-ionization-time-of-flight mass spectrometry (MALDI-TOF MS). The MALDI-TOF MS were carried out using standard ethanol-formic acid extraction method. Results: Twenty five cases from these 95 cases were detected by conventional methods and API-Staph kit to be due to Staphylococcus aureus. All Staphylococcus aureus isolates with 4 Staphylococcus aureus reference strains (control positive) and one E. coli isolate revealed from bovine mastitic case (control negative) were examined using MALDI-TOF MS. The MADI-TOF MS identified all clinical bacterial samples as Staphylococcus aureus with the exception of the control negative sample which was E. coli with 100% agreement with bacteriological and phenotypical examination. Also, MALDI-TOF MS gave a valid score of 100% when used in identification of tested Staphylococcus aureus, control positive and control negative samples with 100% sensitivity in comparison to results obtained by ABI system and conventional methods. Conclusion: This study concluded that according to its fast, accurate and reliable nature, MADLI-TOF MS could be used as alternative diagnostic tool for routine differentiation and identification of Staphylococcus aureus isolates in the clinical bacteriological laboratory. The MALDI-TOF MS need more verification and validation using more samples to detect reliability, sensitivity and performance of this type of bacterial identification.

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