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International Journal of Dairy Science
Year: 2010  |  Volume: 5  |  Issue: 1  |  Page No.: 1 - 9

Optimization of Fermentation Conditions for Bacteriocin Production by Lactococcus lactis CCSULAC1 on Modified MRS Medium

S. Sharma, A.P. Garg and G. Singh    

Abstract: The present study was carried out to optimized medium composition and culture conditions to enhance the bacteriocin production from Lactococcus lactis CCSULAC1 in MRS medium. The optimum temperature and initial pH for bacteriocin production was 7.5 and 35°C, respectively. Lactococcus lactis CCSULAC1 displayed the highest bacteriocin activity when grown in modified MRS medium containing soya extract (SEMRS) as nitrogen source instead of other selective constituents. Different culture media including MRS with 1.5% tween, TGEA, TGYA, Elliker’s media were also studied for bacteriocin production. The bacteriocin showed broad antimicrobial spectrum determined by well diffusion assay against E. coli, Enterobacter sp., S. aureus, Pseudomonas sp., B. polymyxa, B. subtilis, S. typhii, Micrococcus sp. but shown no effect on Candida albicans, Shigella sp., Klebsiella sp. and S. paratyphii. Replacement of soya extract with yeast extract demonstrated the better yield of bacteriocin. The modified SEMRS is a cost effective medium that can be helpful for large scale industries.

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