Comparison of the Cellular Glutathione Levels in Response to Cisplatin in Different Cell Lines
In this study, we have evaluated the effects of cisplatin on the cellular total GSH level in different tumor and normal cell lines. Five different cell lines of human hepatic carcinoma (HePG2), human lung adenocarcinoma (A549), human ovarian carcinoma (SKOV3), dog kidney (LLCPK1), Chinese hamster ovary (CHO) and Human gingival fibroblast (GHF1) cell lines were exposed to their respected IC50 concentrations of cisplatin for two hours. cisplatin cytotoxicity was measured using clonogenic assay and the total cellular GSH level was analyzed using a photometrical assay. The results showed that cisplatin had different degrees of cytotoxicities on different cell lines as shown by IC50 values; o.87 for HepG2, 3.27 for A549, 0.99 for SKOV3, 5.50 for LLCPK1, 5.50 for CHO and 1.60 for GHF1 cell lines and GSH level alterations after exposure to cisplatin were also different for different cell lines that 85.33 for HepG2, 637.00 for A549, 2691.00 for SKOV3, 1388.30 for LLCPK1, 412.60 for CHO and 783.24 for GHF1 cell lines. This study showed that the cellular GSH level increased in LLCPK1, A549, SKOV3 and GHF1 cell lines, but decreased in CHO and HepG2 cell lines versus to the matched controls. The highest significant variation of GSH in cancer cell line was belonging to SKOV3 and in normal cell lines for LLCPK1, after treated with cisplatin. It is concluded that the total GSH variation after exposure to cisplatin is different for different cell lines. We were not able to correlate between the level of resistance to cisplatin (based on the IC50 levels) and GSH level or variations in this study. It might indicate the GSH neither the unique, nor the most important mechanism of resistance to cisplatin in these cell lines, in spite of many publications in its favor.
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