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International Journal of Cancer Research
Year: 2005  |  Volume: 1  |  Issue: 2  |  Page No.: 94 - 100

Clinical Significance of Serum p53 Antigen in Patients with Hepatocellular Carcinoma

Mohamed Mohamed Abdel-Aziz, Nabil Ali Gad El-hak and Ayman Tallatt Abbas    

Abstract: The aim of this study was to analyse the serum p53 antigen concentrations in 98 patients with hepatocellular carcinoma (HCC) using ELISA based on mouse anti-p53 monoclonal antibody (Bp53-12 mAb). A single band of 53 kDa was detected in sera of selected HCC patients using western blot assay. The ELISA technique revealed that the mean serum concentrations of p53 antigen in HCC patients was significantly higher than in controls (p<0.0001). The positive serum of p53 protein (>0.212 ng mL-1) samples were found in 60 out of 98 (61.22%) patients with HCC. There were no significant differences in serum p53 protein levels according to age, sex, or serum AFP, but serum levels of p53 protein in patients with smaller tumor size (<5 cm) were significantly higher than those in patients with larger tumor size (>5 cm) (p = 0.03) . There were significant differences in p53 protein level according to Child Pugh classification (Child A vs. combined Child B and C) (p = 0.011). There was no significant association between serum p53 protein concentrations and serum AFP levels. Positive status of serum p53 protein and/or AFP were found in 57 out of 69 (82.6%) patients with HCC. These results suggest that the quantification of soluble p53 in serum may be an additional useful biochemical marker for immuno-diagnosis of patients with HCC using ELISA as a non-invasive technique.

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