Abstract: Sepsis is a systemic host response which implicates an inflammatory state due
to a bacterial infection and involving multiple organ failure. In order to modulate
this systemic reaction, liver plays a pivotal role due to its central contribution
in metabolism and host defense mechanism. The process of sepsis as an inflammatory
condition results in the production of free radicals, in some cases induced
by lipopolysaccharide which conducts to oxidative damage and mitochondrial impairment;
these alterations may contribute to the pathophysiology of septic shock. The
use of antioxidants in the treatment of this pathology reduces mitochondrial
damage and oxidative stress, therefore in this study, treatment with Nutrisim©
a nutritive supplement used empirically in the treatment of several degenerative
disorders was tested in biochemical parameters indicative of oxidative stress
and mitochondrial ATPase activity in rat liver mitochondria damage-induced by
LPS. The results showed that treatment with Nutrisim©
30 min prior or after the LPS challenge significantly attenuates the end-products
of lipid oxidation (p<0.05), restores total antioxidant capacity, the membrane
fluidity and preserves ATPase activity. In conclusion, NutriSim©
administration prior or after LPS damage, ameliorates liver damage. Therefore,
the beneficial effects of Nutrisim©
against oxidative stress and ATPase activity in rat liver mitochondria presented
in this study may suggest a potential chemoprotective effect in sepsis prevention. |