Abstract: Sepsis is a systemic host response which implicates an inflammatory state due to a bacterial infection and involving multiple organ failure. In order to modulate this systemic reaction, liver plays a pivotal role due to its central contribution in metabolism and host defense mechanism. The process of sepsis as an inflammatory condition results in the production of free radicals, in some cases induced by lipopolysaccharide which conducts to oxidative damage and mitochondrial impairment; these alterations may contribute to the pathophysiology of septic shock. The use of antioxidants in the treatment of this pathology reduces mitochondrial damage and oxidative stress, therefore in this study, treatment with Nutrisim^© a nutritive supplement used empirically in the treatment of several degenerative disorders was tested in biochemical parameters indicative of oxidative stress and mitochondrial ATPase activity in rat liver mitochondria damage-induced by LPS. The results showed that treatment with Nutrisim^© 30 min prior or after the LPS challenge significantly attenuates the end-products of lipid oxidation (p<0.05), restores total antioxidant capacity, the membrane fluidity and preserves ATPase activity. In conclusion, NutriSim^© administration prior or after LPS damage, ameliorates liver damage. Therefore, the beneficial effects of Nutrisim^© against oxidative stress and ATPase activity in rat liver mitochondria presented in this study may suggest a potential chemoprotective effect in sepsis prevention.