Abstract: Diseases caused due to ‘oxidative stress’ initiated by Reactive Oxygen Species (ROS) is growing at an alarming rate. Several medicinal herbs explored till date were reported to possess numerous pharmacological properties used for combating different diseases. The present study was undertaken to investigate the inhibitory potential of Terminalia chebula by H₂O₂ induced oxidative stress in THP-1 cell line and also the presence of the marker compounds in T. chebula was confirmed by High Performance Liquid Chromatography (HPLC). Intracellular quantification of ROS was done using a flow cytometer. The percentage of gallic acid and ellagic acid in the extract was performed by HPLC were found to be 4.84% w/w and 2.56% w/w, respectively. Pretreatment of cells with T. chebula extract significantly reduced the intracellular ROS production in a dose-dependent manner. The present study illustrates the effective role of T. chebula in reducing the ROS production which is a causative factor for several disorders. The results describe the possibility and potentiality of natural sources to inhibit the pathogenesis of different diseases caused due to oxidative stress.