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International Journal of Botany

Year: 2007  |  Volume: 3  |  Issue: 4  |  Page No.: 390 - 393

Efficient Adventitious Shoot Regeneration from Root Explants of Aralia elata Seem

M.Z. Karim, S. Yokota, M.M. Rahman, J. Eizawa, Y. Saito, M.A.K. Azad, F. Ishiguri, K. Iizuka and N. Yoshizawa

Abstract

A highly reproducible in vitro shoot regeneration system in Aralia elata Seem. using root explants was developed in this investigation. Multiple shoots were induced in vitro directly from root explants through adventitious shoot bud regeneration. The ability of root explants to produce shoot buds depended on the supplementation of Plant Growth Regulators (PGRs). Maximum multiplication of shoots (18 shoots per explant) was achieved in a Broad-leaved Tree (BT) medium supplemented with 1.00 μM 6-benzyl aminopurine (BAP). The best and healthiest rooting was observed in a BT medium supplemented with 2.00 μM μ-naphthaleneacetic acid (NAA). Regenerated plants were successfully acclimatized under ex vitro condition.

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