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Year: 2007 | Volume: 3 | Issue: 3 | Page No.: 296 - 301
Neelofar Jabeen, A.S. Shawl, G.H. Dar, Arif Jan and Phalesteen Sultan
Abstract
A rapid in vitro regeneration protocol for induction of multiple shoots from nodal segments of Inula racemosa Hook.f. was developed. Leaf and nodal segments were inoculated on MS medium containing different concentrations of Benzylaminopurine (BAP) either alone or in combination with Naphthalene Acetic Acid (NAA) or Indole Butyric Acid (IBA) growth hormones. MS medium supplemented with BAP (0.25 mg L–1) induced maximum number of shoots (20.7±0.8). The shoots were rooted on half strength of MS medium either alone or supplemented with (IBA) Indole butyric acid (1.0 mg L–1). Direct rooting from leaves has also been developed from cultured plants. In vitro raised plantlets were acclimatized in green house and successfully transplanted to the field with a survival of 80%.