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International Journal of Botany
Year: 2005  |  Volume: 1  |  Issue: 1  |  Page No.: 19 - 22

Cotton Saccharifying Activity of Cellulases by Trichoderma harzianum UM-11 in Shake Flask

Ikram-ul-Haq, Uzma Hameed, Kiran Shahzadi, M. Mohsin Javed, Sikander Ali and M.A. Qadeer    

Abstract: The present study deals with the optimization of cultural conditions for Cotton Saccharifying Activity (CSA) of cellulases by Trichoderma harzianum UM-11 using shake flask technique in 250 mL Erlenmeyer flasks. Among the different fermentation media tested, Carboxymethyl Cellulose (CMC) with Mineral Salts Solution (MSS) gave the best CSA. Cotton saccharification was optimal when 25 mL of the medium was incubated at 28°C for 96 h. The maximum CSA (0.528 U/mL/h) was found at initial pH 6.0. The level of inoculum was optimized at 5.0x107 conidia/25 mL medium.

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