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International Journal of Agricultural Research
Year: 2013  |  Volume: 8  |  Issue: 1  |  Page No.: 17 - 25

Genetic Discrimination of Egyptian Fig Cultivars Revealed by RAPD Fingerprints

Nabil Sabet A. Mustafa and Mohamed Abou-Ellail    

Abstract: Ficus carica L., well adapted to the Mediterranean climate, is ubiquitous in Egypt. In spite of the large possibilities of its adaptation to the Egyptian climate, its cultivation remains traditional. In Egypt, these species is represented by a large number of varieties which are facing genetic erosion. To save these genetic resources some of the Egyptian varieties had been studied using molecular markers. Nine RAPD markers were used to survey the genetic diversity in Egyptian fig (Ficus carica L.). Seven fig cultivars originating from diverse geographical areas were analyzed. As a result, considerable genetic diversity was detected among the studied F. carica accessions. The relationships among the seven varieties were studied by cluster analysis. The dendrogram showed two main groups composed of cultivars. Our data proved that RAPD markers are useful for germplasm discrimination as well as for investigation of patterns of variation in fig. Since this designed procedure has permitted to establish a molecular database of the reference collections, the opportunity of this study is discussed in relation to the improvement and rational management of fig germplasm. Results indicated that RAPD is useful, rapid and accurate technique for studying genetic diversity and germplasm characterization of Ficus carica some cultivars. There is a wide spectrum genetic variation among studied fig varieties, these variation could be an effective factor in breeding program.

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