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International Journal of Agricultural Research
Year: 2011  |  Volume: 6  |  Issue: 1  |  Page No.: 40 - 48

Micropropagation of an Elite Medicinal Plant: Stevia rebaudiana Bert.

Arpita Das, Saikat Gantait and Nirmal Mandal    

Abstract: To sustain the supply of quality propagules, the present study was carried out to develop a novel protocol for accelerated in vitro mass multiplication in stevia (Stevia rebaudiana Bert.) through multiple shoot induction using shoot tips, nodal segments and axillary bud explants. The earliest bud induction was recorded from the shoot tip explants within 6 days of culture in Murashige and Skoog (MS) medium, in comparison to the other two explants. MS basal medium supplemented with sucrose (30 g L-1), agar (7 g L-1) and kinetin (2 mg L-1) performed best in multiple shoot proliferation, resulting more than 11 multiple shoots from a single shoot tip explant within 35 days of culture. For root induction and elongation, MS medium devoid of plant growth regulator performed most dynamically, where MS medium plus indole-3-acetic acid and 6-benzylaminopurine showed an adverse effect, promoting undesirable callus growth at root zone. In vitro generated propagules were successfully acclimatized in a balanced mixture of sand, soil and farm yard manure (1:1:1 v/v). Peroxidase assay along with ISSR fingerprinting confirmed the genetic clonality of in vitro generated propagules.

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