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International Journal of Agricultural Research
Year: 2009  |  Volume: 4  |  Issue: 1  |  Page No.: 38 - 45

Isolation and Characterization of A Novel Glu-Bx HMW-GS Allele from Tibet Bread Wheat Landrace

Zehong Yan, Shoufen Dai, Dengcai Liu, Yuming Wei, Jirui Wang and Youliang Zheng    

Abstract: A novel HMW-GS of Bx6** , with slightly slower migration rate than that of Bx7 presented in wheat cultivar Chinese Spring, was found in a Tibet bread wheat landrace using SDS-PAGE. The gene for this subunit was isolated and its sequence was obtained. This gene was very similar to Bx7 both in nucleotide and deduced amino acid sequence. At the nucleotide sequence level, Bx6** different from Bx7 by the deletion of an 18 bp fragment and three nucleotides replacement at position 455 A/G, 2046 G/A and 2208C/G, respectively. At the deduced amino acid sequence level, the only difference is that Bx6** shorter than Bx7 by the deletion of a hexaploid peptide unit (PGQGKQ). These results suggested that Bx6** was a derivation of Bx7 and was formed by replication slippage.

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