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International Journal of Agricultural Research
Year: 2009  |  Volume: 4  |  Issue: 3  |  Page No.: 131 - 136

Karyotype Studies on Pseudoroegneria gracillima and P. kosaninii (Poaceae: Triticeae)

Haiqing Yu, Chunbang Ding, Chun Zhang and Yonghong Zhou    

Abstract: In order to obtain more cytological data, the karyotypes of Pseudoroegneria gracillima and P. kosaninii were investigated. Root tips of P. gracillima and P. kosaninii were pretreated in an ice bath, fixed in a mixture of 95% ethanol: glacial acetic acid and treated in 1 M HCl at 60°C in a water bath. Somatic cells were stained in Schiff at room temperature and the meristematic portions of the root tips were squashed in 45% acetic acid. The results show that: (1) P. gracillima is diploid with two pairs of satellites and P. kosaninii is octoploid with three pairs of satellites. The karyotypes of diploid P. gracillima and octoploid P. kosaninii are first reported, (2) the karyotype formulas of P. gracillima and P. kosaninii are 2n = 2x = 14 = 12 m (2sat)+2sm (2sat) and 2n = 8x = 56 = 42 m (6sat)+12sm+2st, respectively and (3) the karyotype of P. gracillima is 1A type, while P. kosaninii is 2B type. This demonstrated that there are great variations between the karyotypes of P. gracillima and P. kosaninii.

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