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International Journal of Agricultural Research
Year: 2008  |  Volume: 3  |  Issue: 2  |  Page No.: 161 - 165

Production of Bacteriocins by Root Nodule Bacteria

M. Sridevi and K.V. Mallaiah    

Abstract: Twenty Rhizobium species were isolated from root nodules of 20 legume hosts and studied for their ability to produce bacteriocins. Among the 20 species, the Rhizobium sp. isolated from root nodules of Crotalaria alata, C. juncea and C. laburnifolia produced bacteriocins after 72 h of growth. It was observed that Rhizobium sp. from C. alata produced small type and highly effective bacteriocin against Rhizobium sp. from Arachis hypogaea, Cajanus cajan, Clitorea ternatea, Dolichos lablab, Indigofera linnaei and Vigna mungo. The bacteriocin was isolated and partially purified by chloroform extraction and ammonium sulphate fractionation. The bacteriocin was sensitive to protease and insensitive to DNase and RNase. It was stable up to temperature 80 °C for 15 min. The bacteriocin fraction consistently migrated as a 29 kDa polypeptide on SDS-PAGE.

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