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International Journal of Agricultural Research
Year: 2007  |  Volume: 2  |  Issue: 5  |  Page No.: 468 - 475

Callus Induction from Leaf Explants of Ficus deltoidea Jack

Anna Ling Pick Kiong, Then Chia Nee and Sobri Hussein    

Abstract: Murashige and Skoog (MS) nutrient media supplemented with auxins 2,4-dichlorophenoxyacetic acid (2,4-D), Naphthaleneacetic acid (NAA), Indole-3-acetic acid (IBA) and 4-amino-3, 5, 6-trichloro picolinic acid (picloram) each at the concentrations of 1, 3 and 5 mg L-1 were evaluated for their effects on the callus induction from the leaf explants of Ficus deltoidea. The best calli with healthy morphology and growth was obtained from explants cultured on MS media supplemented with 3 mg L-1 picloram. Friable calli with white, greenish or yellowish colour were obtained after 10 days upon culturing. 2, 4-D and IBA induced morphologically unhealthy calli, whereas cultured explants gave a negative response to MS media supplemented with various levels of NAA. For callus maintenance, the study indicated that media supplemented with 1 mg L-1 picloram gave the best growth rate of calli and was the most suitable callus maintenance medium to be used.

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