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International Journal of Agricultural Research
Year: 2006  |  Volume: 1  |  Issue: 2  |  Page No.: 183 - 193

Adventitious Shoots Regeneration from Root and Stem Explants of Eurycoma longifolia Jack - An Important Tropical Medicinal Plants

Sobri Hussein, Rusli Ibrahim and Anna Ling Pick Kiong    

Abstract: This study reported the first successful adventitious shoots regeneration from the root and stem explants of E. longifolia. Generally, stem explants appeared to have a lower regeneration capability when treated with the cytokinins alone compared to the root explants. In the root explant, the best medium formulation determined was basal Juglans medium (DKW) supplemented with 1.0 mg L-1 of kinetin + 1.0 mg L-1 of zeatin whereas in the stem explants, basal woody plant medium (WPM) enriched with 2.0 mg L-1 of BAP + 2.0 mg L-1 of zeatin was found to be the best medium formulation in increasing the regeneration rate. Stem and root explants that were 2.0 cm distant from one another has been identified as the most suitable position for attaining the maximum percentage of adventitious shoots formation. Rootlets induction of adventitious shoots formed was also achieved and the highest induction rate was attained in basal MS medium supplemented with 0.5 mg L-1 of Indole-3-butyric acid. Acclimatization of the in vitro plantlets produced survived well with no morphological differences from the parent plants after two months of transplantation to the soil.

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