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International Journal of Environmental Analytical Chemistry
Year: 2009  |  Volume: 89  |  Issue: 1  |  Page No.: 59 - 66

Determination of carbofuran by the quenching effect on resonance light scattering and its application to water and vegetable samples

Shicong Hou, Yan Cui, Fengpei Du, Weifu Kong, Junfen Song, Ying Li and Nianqin Jie    

Abstract: A new method for the determination of carbofuran (CF) with DNA by a resonance light scattering (RLS) technique was developed. The intensity of RLS (IRLS) of DNA-HCl system was significantly quenched in presence of CF. A RLS peak at 315.6 nm was found, and the quenched intensity of RLS was proportional to the concentration of CF. The linear range of the calibration curve was ≈0.02-2.0 μg mL-1 and the detection limit (S/N = 3) 7 ng mL-1. The CF in river water, cucumbers and rice samples was determined. The recovery rates were in the range of 90.0-111.1, 95.0-106, 93.0-111.0%, respectively. The mechanism of the reaction between CF and DNA is also discussed.

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