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Bulletine of UASVM Animal Science and Biotechnologies
Year: 2010  |  Volume: 67  |  Issue: 1  |  Page No.: 492 - 492

The Influence of Freezing on Some Rare Breeds Boar Semen Cryopreservation

Marius ZAHAN, Vasile MICLEA, Florin GHIURU, Iulian ROMAN, Alexandru RUSU, Ileana MICLEA and Manuel MIHAILESCU    

Abstract: Despite the new researches in boar semen cryopreservation, application of frozenthawed (FT) semen in commercial artificial insemination (AI) programs is still limited. The main sources of semen used in AI are semen preserved on room temperature (RT) and sometimes fresh (F) semen. However, the use of male germplasm from genebank on some rare breeds is connected with the ability to use frozen semen. The aim of this study was to evaluate the effect of semen preservation method and especially the influence of freezing on Red Mangalitsa and Bazna boar semen cryopreservation. Six sexually mature boars from Red Mangalitsa and Bazna rare breeds of the Faculty of Animal Sciences and Biotechnology, USAMV Cluj-Napoca, were used in this study. Semen was collected using the gloved-hand method, evaluated for conventional semen characteristics and extended in Beltsville Thawing Solution (BTS). Preservation was made by RT and by frozen using a modified method described by Westendorf et al. (1975), in lactose, egg yolk, glycerol and Orvus ES Paste extender (LEYGO). The F, RT and FT sperm was evaluated by Eosin-nigrosin viability staining, acrosomal integrity shown by normal apical ridge (NAR) and plasma membrane integrity by hypo-osmotic swelling test (HOST) in order to establish sperm quality. Because it is known that the life of sperm is short in utero after thawing, the FT sperm quality was also determined by thermoresistance test (motility at 10, 30, 60, 120 and 240 minutes). The results were statistically analyzed using ANOVA. The first parameter affected by freezing was viability, but the state of the acrosome has shown significant differences between the preservation methods on both breeds. In comparison with F and RT semen the value of NAR was halved. In addition, membrane integrity spermatozoa (HOST) was significantly lower after the FT procedure compared to F and RT. Thermoresistance test has shown a good motility until 120 minutes, with the best value at 30 minutes, but decreased dramatically at 240 minutes. However, this results correlated with the reducing insemination-to-ovulation interval could improve the fertility after AI with Red Mangalitsa and Bazna breeds’ semen cryopreservation

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