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Bulletine of UASVM Animal Science and Biotechnologies
Year: 2010  |  Volume: 67  |  Issue: 1  |  Page No.: 445 - 451

Antioxidant Medium for Mangalita Boar Semen Cryopreservation

Florin VARO GHIURU, Ioan LADOSI, Iulian ROMAN, Andrea HETTIG, Marius ZAHAN and Vasile MICLEA    

Abstract: The technical establishment of boar sperm cryopreservation is indispensable for effective breeding of the Mangalita native pig. The objective of the present study was to determine whether ascorbic acid is capable of improving the quality of cryopreserved Mangalita spermatozoa. Ejaculated Mangalita and PIC (as comparison) boar sperm frozen in an extender supplemented with 200μM ascorbic acid (vitamin C), 400μM Trolox® (a vitamin E hydrosoluble analogue), 200+400μM vit.C+vit.E, 200+200μM vit.C+vit.E, or without supplementation was thawed and then evaluated the sperm motility. Treatment with 200+400μM vit.C + vit.E has the most beneficial effect on the Mangalita sperm motility after frozen-thawing among the concentrations tested (P<0.05).

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