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Bulletine of UASVM Animal Science and Biotechnologies
Year: 2010  |  Volume: 67  |  Issue: 1  |  Page No.: 409 - 413

The Influence of Lutein on Swine Embryo Development

Ileana MICLEA, Marius ZAHAN, Vasile MICLEA, Andrea HETTIG, Iulian ROMAN, Florin GHIURU and Elena ILISIU    

Abstract: As defence against oxidative stress, living systems employ antioxidants that they either produce or take up from the environment. Among these lutein has been shown to be a powerful antioxidant that also functions as a signal molecule. The goal of this study was to establish the influence of several lutein concentrations on swine embryo development in vitro, in order to improve culture media. Pig oocytes were cultured for 45 hours at 37°C in 5% CO2 atmosphere; in M199 containing several lutein (2.5, 4, 5, 8, and 10 μM) concentrations. Then, they were fertilized in TALP medium using spermatozoa capacitated by centrifugation and incubated for 16-18 hours. Afterwards, the presumed zygotes were cultured in NCSU-23 droplets supplemented with lutein (2.5, 4, 5, 8, and 10 μM) in the same conditions. Their development was assessed at 48 and 120 hours. The number of embryos that had developed to the 2 cells, 4-8 cells and morula stages was compared to the control, and the differences analyzed using the analysis of variance and interpreted using the LSD and Duncan tests. Lutein (2.5 μM) supplementation has a beneficial effect on embryo development to the morula stage. This is apparent at 48 hours as well as 120 hours.

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