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Bulletine of UASVM Animal Science and Biotechnologies
Year: 2010  |  Volume: 67  |  Issue: 1  |  Page No.: 403 - 408

Influence of Alpha-tocopherol on Swine Embryo Development

Ileana MICLEA, Marius ZAHAN, Vasile MICLEA, Andrea HETTIG, Iulian ROMAN and Florin GHIURU    

Abstract: As defence against oxidative stress, living systems employ antioxidants that they either produce or take up from the environment. Among these α-tocopherol has been shown to improve swine oocyte maturation and the development of bovine and swine embryos. The goal of this study was to establish the influence of several α-tocopherol concentrations on swine embryo development in vitro, in order to improve culture media. Pig oocytes were cultured for 45 hours at 37°C in 5% CO2 atmosphere; in M199 containing several α-tocopherol (5, 10, 20, 40 and 80 μM) concentrations. Then, they were fertilized in TALP medium using spermatozoa capacitated by centrifugation and incubated for 16-18 hours. Afterwards, the presumed zygotes were cultured in NCSU-23 droplets supplemented with α-tocopherol (5, 10, 20, 40 and 80 μM) in the same conditions. Their development was assessed at 48 and 120 hours. The number of embryos that had developed to the 2 cells, 4-8 cells and morula stages was compared to the control, and the differences analyzed using the analysis of variance and interpreted using the LSD and Duncan tests. At 48 hours after fertilization it was apparent that α-tocopherol supplementation increased embryo development to the 4-8 cell (10, 20, 40, 80 μM) and morula (5, 40, 80 μM) stages. At 120 hours even more embryos had reached the morula stage.

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