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Bulletine of UASVM Animal Science and Biotechnologies
Year: 2010  |  Volume: 67  |  Issue: 1  |  Page No.: 36 - 42

Determination of Chloramphenicol in Honey by Liquid Chromatography-Mass Spectrometry with Photodiode Array Detection

Liviu Al. MARGHITAS, Victorita BONTA, Daniel DEZMIREAN and Otilia BOBIS    

Abstract: The aim of the present work was the development, validation and implementation in routine analysis of a technique that guarantees low detection limit for chloramphenicol in honey using liquid chromatography (LC)-mass spectrometry (MS) with photodiode array (PDA) detection. Sample preparation was carried out by liquid-liquid extraction of a honey solution in phosphoric buffer (PBS, pH=7.8). The determinations were performed using a Zorbax Eclipse XDB-C18 column and gradient mobile phase, using an electrospray ionization source (ESI) in negative ion mode. Recoveries were calculated at three concentration levels and higher values than 85% were obtained, with relative standard deviations less than 7.7%. The applicability of the present method was tested on 12 honey samples purchased from different beekeepers from Transylvania.

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