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Bulletine of UASVM Animal Science and Biotechnologies
Year: 2010  |  Volume: 67  |  Issue: 1  |  Page No.: 313 - 320

Biochemical and Cytological Investigations on Haemolymph of Apis Mellifera Carpathica Bee in Stressful Conditions

Agripina SAPCALIU, Crenguţa PAVEL, Vasilica SAVU, Eliza CAUIA, Mioara MATEI and Ion RADOI    

Abstract: The study presents changes of biochemical parameters and cytological profile in the haemolymph of Apis mellifera Carpathica bee in stressful conditions compared with aspects in haemolymph of natural living bees. Investigations were made in 2009 on a total of 20 colonies under stress factors and 176 colonies of healthy bees. Harvesting and processing of haemolymph fresh samples from both groups were followed by analysis via classical biochemistry and cytological examination. In stressed bees haemolymph were found significant increases in alkaline phosphatase (ALP), aspartataminotransferaze (AST), gammaglutamiltranspeptidaze (GGT), creatinine (Cre), total calcium (Ca), triglycerides (TG) and significant reduction of magnesium (Mg), compared with values in natural living bees. The others analyzed biochemical parameters (GPT/ALT, CPK, GLU, T-Pro, UA, Urea) showed no significant differences between stressed bees and natural living bees. Monitoring the metabolic status of bees in stress conditions requires only evaluation of a few biochemical parameters (ALP, AST, GGT, total Ca, Mg, Cre and TG). The cytological investigation showed differences in the proportion of haemocyte types between the 2 groups of natural living honeybees and the 2 groups of bees kept in stressful conditions. It was observed an inversion of proportion between plasmatocyte cells (PL) and granulocyte cells (GR), with increased percent of PL and prohaemocyte cells (PRO) in the haemolymph of stressed bees, wile oeonocyte (OE) and other cells showed no important variations. The cytological profile of bees in stressful conditions shows the importance of monitoring the differentiate haemocyte count. Biochemical investigations correlated to the cytological ones more accurately reflect the transformations that occur under the action of stressors in the bee haemolymph.

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