Asian Science Citation Index is committed to provide an authoritative, trusted and significant information by the coverage of the most important and influential journals to meet the needs of the global scientific community.  
ASCI Database
308-Lasani Town,
Sargodha Road,
Faisalabad, Pakistan
Fax: +92-41-8815544
Contact Via Web
Suggest a Journal
Bulletine of UASVM Animal Science and Biotechnologies
Year: 2010  |  Volume: 67  |  Issue: 1  |  Page No.: 260 - 265

The Influence of the Dilution Rate and Glycerol Concentration on Ram Semen Preservation

Vasile MICLEA, Anca DASCAL-CIORNEI, Marius ZAHAN, Vasile DASCAL-CIORNEI and Ileana MICLEA    

Abstract: The goal of our research was to establish the semen quality of some rams from the Tsigai breed, the hill ecotype and itís suitability to conservation thru refrigeration. After the examination of the fresh semen, ejaculates with 0.9-1.0 mobility were further diluted with Triladyl and another extender prepared in hour laboratory named G Extender. 2.5%, 5%, 7%, 10% and 12.5% Glycerol was added to the G Extender. The fresh semen was diluted with 1:1, 1:2, 1:3 and 1:4 ratio with the two diluents with different glycerol concentrations, and further refrigerated. The fresh semen was diluted at 370C. The temperature was decreased to 50C within 2 hours. Periodically mobility determination was performed to al experimental trials. Dropping the temperature to 50C has little influence on the semen mobility. Major differences between trials regarding the spermatozoa mobility appeared when the semen was conserved at 40C, and the best results were obtained when the G Extender with 7% glycerol is used. The 10% and 12.5% glycerol concentration provided the lower results. Regarding the dilution rate, both Triladyl and Extender G gave the best results when the semen was diluted 1:3 and 1:4. The semen prepared with the 1:4 dilution with 7% glycerol in the G Extender maintained the insemination quality 480 hours.

View Fulltext    |   Related Articles   |   Back
   
 
 
 
  Related Articles

No Article Found
 
 
 
Copyright   |   Desclaimer   |    Privacy Policy   |   Browsers   |   Accessibility