Asian Science
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Explore the Effects of Fertilization on Polyphenols Content in Autumn of
Forages from Hill Permanent Grassland by Principal Component &
Classifications Analysis
Abstract: Principal Components and Classification Analysis (PC&CA) represents one of the
most utilised multivariate chemometric techniques, having the advantage to use many measurements
for a single sample in the same time, being recommended for understanding better the complexity of
one phenomenon. The aim of this paper was to use PC&CA to study the effects of different types of
fertilizers on polyphenols content of forages harvested in autumn from permanent grassland.
Gravimetrically was established the matrix of floristic composition. The experimental field was
fertilized first time in 2003, organic and/or NPK mineral. The determination of polyphenols contents
was made using UV-VIS SPECORD 205 spectrophotometer, in conformity to chemical Folin and
Ciocalteu colorimetric method. The highest polyphenols content was identified in forages from
unfertilized variant (108 μM gallic acid/g). PC&CA can be a useful tool in describing the modification
of polyphenols contents of forages under the effects of organic and/or mineral fertilisation of
permanent grassland.