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Biotechnology
Year: 2014  |  Volume: 13  |  Issue: 5  |  Page No.: 239 - 244

Encapsulation of Biosurfactant-Producing Bacillus licheniformis (PTCC 1320) in Alginate Beads

Mandana Ohadi, Bagher Amir-Heidari, Mohammad Hassan Moshafi, Ali Mirparizi, Mohammadzaman Basir and Gholamreza Dehghan-Noudeh    

Abstract: In the present study, biosurfactant production by immobilized Bacillus licheniformis PTCC 1320 in alginate beads has been studied. Alginate solution and bacterial suspension were mixed and stirred to get a uniform mixture. The slurry was taken into a sterile syringe and added drop wise into a stirring calcium chloride solution from a 5 cm height to form spherical beads. The beads were kept under agitation for about 2 h to harden and were then washed. Equal numbers of beads were charged to 250 mL erlenmeyer flasks each containing 50 mL of nutrient broth medium. Beads containing B. licheniformis placed in the nutrient broth medium were incubated in a shaker incubator at 30°C. Samples were collected at 24 h intervals and the parameters of biosurfactant production, such as surface tension, emulsification activity and foam production were studied. Results showed that B. licheniformis entrapped in calcium alginate beads is able to preserve its viability and produce biosurfactant as a secondary metabolite. Maximum biosurfactant production was achieved during the first 24 h.

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