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Year: 2014  |  Volume: 13  |  Issue: 4  |  Page No.: 190 - 195

Application of in silico PCR Strategy for Primer Design and Selection of Chicken AMPK Gamma Subunit Gene Loci

Wu-Yi Liu    

Abstract: Polymerase Chain Reaction (PCR) is a fundamental in vitro technique to molecular biology in practice. However, the utility of PCR and PCR-based methods is dependent on the unique of identifying and designing and efficient primer sequences. In silico PCR can thus assist in selection of newly designed primers, identify potential mismatches in the primer binding sites and greatly avoid the amplification issues of unwanted amplicons before any real experiments in practice. The present study was aimed to design and select novel primers and validate reported primers for multi-exon genes in practical investigation procedure on the Single Nucleotide Polymorphism (SNP) of PRKAG2 and PRKAG3 gene loci in Chinese native chicken populations applying in silico PCR analysis strategy. After careful experimental operation and comparison, it was found that results of in silico PCR analysis and the real experimental PCR amplifications were strikingly in resemblance.

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