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Biotechnology
Year: 2011  |  Volume: 10  |  Issue: 6  |  Page No.: 514 - 520

DNA Fragmentation, Linear Velocity and Fertilising Ability of Reactivated Cryopreserved Goldfish Sperm using Different Cryoprotectants

C. Nathanailides, T. Chanzaropoulos, A. Barbouti, C. Perdikaris and T. Zhang    

Abstract: The motility, fertilising ability and DNA integrity of freeze-thawed goldfish sperm was evaluated using computer-aided sperm motility analysis and the comet assay. The results indicate that sublethal effects during the freeze-thawing procedure compromised sperm fertilising ability, DNA integrity, velocity linearity and the duration of swimming. Compared to 10% Ethylene glycol (EG), 5% and 10% Egg yolk (EY) and DMSO treated sperm exhibited higher percentage of motile sperm and higher path velocity (VAP); straight-line velocity (VSL) linearity (LIN), DNA integrity and fertilising ability. There was a low but significant (r = 0.67, p<0.05) correlation between DNA integrity and the maximum Duration of Forward Progressive Swimming (DFPS) ability of cryopreserved goldfish sperm. DNA integrity and to a lesser degree the simple assessment of the DFPM of thawed sperm may be reliable indicators of sperm integrity for the development and the rapid assessment of fish sperm cryopreservation protocols.

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