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Biotechnology
Year: 2010  |  Volume: 9  |  Issue: 1  |  Page No.: 84 - 88

Ethanol Production from Cassava Starch by Selected Fungi from an-Koji and Saccaromycetes cereviseae

Thalisa Yuwa-Amornpitak    

Abstract: Ethanol production from cassava starch by co-culture of selected fungi and S. cerevisiae was investigated. Firstly starch hydrolysis fungi were isolated and screened from tan-koji (loog-pang). Enzymatic activities of 10 isolated Rhizopus sp. strains were determined on 0.1% starch agar plate at various pH (3-10) as primary screening. Clear zone diameter was occurred at pH ranging from 3 to 8 and no response at pH 10. The highest clear zone diameter that were found from 2 strains of Rhizopus sp. #2Bu and Rhizopus sp. #3Su at pH 4 were nearly equal. Secondary screening, sugar liberated were performed at various starch concentration from these 2 fungal strains. The results showed that Rhizopus sp. #3Su was the maximum efficiency. The highest reducing sugar yield was 25.9% from 6% cassava starch medium at 72 h. Ethanol production by SSF process, the coupling process between saccharification and fermentation was developed by using co-culture of Rhizopus sp. #3Su and Saccharomyces cerevisiae 5088. After 24, 48 and 72 h of saccharification period by the fungal strain, the fermentation process was begun by adding yeast inoculum. The highest ethanol production was achieved at 14.36 g L-1 after 24 h of saccharification process on 6% cassava starch medium.

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